Original Article | Article
"Detection of Hemoglobinopathies by HPLC in a Referral Clinical Laboratory in Nepal "
Abha Shrestha, Vivek Pant, Keyoor Gautam, Devish Pyakurel, Santosh Pradhan
Nepalese Medical Journal

Hemoglobin fraction analysis by ion-exchange in HPLC has the advantage of quantifying HbF and
HbA2 along with hemoglobin variant screening in a single reproducible system making it an excellent tool to screen for hemoglobin variants and hemoglobinopathy.

Tags Applied
Hemoglobinopathies HPLC Nepal Thalassemia


Introduction: Various hemoglobin variants are prevalent in the Nepalese population owing to the ethnic diversity of our population. Detection of asymptomatic carriers by a reliable laboratory
method is the cornerstone of prevention of this serious health problem. The simplicity of the automated system with internal sample preparation, superior resolution, rapid assay time,
and accurate quantification of hemoglobin fractions makes ion-exchange high-performance liquid chromatography (IEX- HPLC) an ideal methodology for the routine screening for
hemoglobinopathies. We report the clinical laboratory-based prevalence of variant hemoglobin and hemoglobinopathies through IEX- HPLC analysis in a cohort of patients referred for hemoglobin
electrophoresis to a referral clinical laboratory in Nepal.
Materials and Methods: The variant hemoglobin and hemoglobinopathy were diagnosed based on percentage, retention time, and peak characteristics of variant hemoglobin in a chromatogram.
Peripheral blood films, reticulocyte count, serum iron profile, and sickling test were done in selected cases along with detailed family history.
Results: Hemoglobinopathy was detected in all age group but the vast majority was detected between 20 to 40 years of age. Beta thalassemia trait was the most frequently detected hemoglobinopathy
in all age groups.
Conclusions: The present study conducted using IEX-HPLC reflects the magnitude of thalassemia and hemoglobinopathies in a laboratory-based population which helps to increase awareness among
both health caregivers and the general population. Routine screening for hemoglobinopathy of individuals at the reproductive age group is recommended and this screening can be done through